MersV1, Main, Exploration, bibRecord, 004E45

Human transforming growth factor-α: Precursor structure and expression in E. coli

Identifieur interne : 004E45 ( Main/Exploration ); précédent : 004E44; suivant : 004E46

Human transforming growth factor-α: Precursor structure and expression in E. coli

Auteurs : Rik Derynck [États-Unis] ; Anita B. Roberts [États-Unis] ; Marjorie E. Winkler [États-Unis] ; Ellson Y. Chen [États-Unis] ; David V. Goeddel [États-Unis]

Source :

Cell [ 0092-8674 ] ; 1984.

RBID : ISTEX:ADED61193096E6480535E2DB96614A73BF1044AF

English descriptors

Teeft :
- Acad, Acid, Acid sequence, Amino, Amino acid, Amino acid differences, Amino acid sequence, Amino acids, Anzano, Assay, Biol, Biological activity, Bromide, Carcinoma, Cdna, Cdna clone, Cdna clones, Cdna sequence, Cell carcinoma, Cell line, Cellular transformation, Clone, Coding, Codon, Coli, Cyanogen, Cyanogen bromide, Epidermal, Epidermal growth factor, Exon, Fragment, Fusion protein, Fusion proteins, Genomic, Glutathione, Goeddel, Growth factor, Growth factors, Homology, Hybridization, Hybridizing, Larco, Marquardt, Massague, Methionine, Mrna, Murine, Nucl, Nucleotide, Nucleotide sequence, Ozanne, Peptide, Phage, Plasmid, Plasmid pte6, Polypeptide, Precursor, Precursor polypeptide, Proc, Pte5, Pte6, Radioreceptor assay, Receptor, Receptor binding, Restriction fragment, Room temperature, Sarcoma, Sequence coding, Short fusion protein, Soft agar, Sporn, Tgfs, Todaro, Trpale, Tumor cells, Twardzik, Ullrich, Yanofsky.

Abstract

Abstract: Transforming growth factor-α (TGF-α) is secreted by many human tumors and can induce the reversible transformation of nontransformed cell lines. Using long synthetic deoxyoligonucleotides as hybridization probes we isolated an exon coding for a portion of TGF-α from a human genomic DNA library. Utilizing this exon as a probe, a cell line derived from a human renal cell carcinoma was identified as a source of TGF-α mRNA. A cloned TGF-α cDNA was isolated from a cDNA library prepared using RNA from this cell line, and was found to encode a precursor polypeptide of 160 amino acids. The 50 amino acid mature TGF-α produced by expression of the appropriate coding sequence in E. coli binds to the epidermal growth factor receptor and induces the anchorage independence of normal mammalian cells in culture.

Url:

https://api.istex.fr/ark:/67375/6H6-3G5MZBT1-9/fulltext.pdf

DOI: 10.1016/0092-8674(84)90550-6

Affiliations:

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Le document en format XML

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<author><name sortKey="Winkler, Marjorie E" sort="Winkler, Marjorie E" uniqKey="Winkler M" first="Marjorie E." last="Winkler">Marjorie E. Winkler</name>
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<author><name sortKey="Chen, Ellson Y" sort="Chen, Ellson Y" uniqKey="Chen E" first="Ellson Y." last="Chen">Ellson Y. Chen</name>
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<term>Amino acid differences</term>
<term>Amino acid sequence</term>
<term>Amino acids</term>
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<term>Fragment</term>
<term>Fusion protein</term>
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<term>Mrna</term>
<term>Murine</term>
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<term>Nucleotide</term>
<term>Nucleotide sequence</term>
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<term>Plasmid</term>
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<term>Precursor polypeptide</term>
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<term>Pte6</term>
<term>Radioreceptor assay</term>
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<term>Receptor binding</term>
<term>Restriction fragment</term>
<term>Room temperature</term>
<term>Sarcoma</term>
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<front><div type="abstract" xml:lang="en">Abstract: Transforming growth factor-α (TGF-α) is secreted by many human tumors and can induce the reversible transformation of nontransformed cell lines. Using long synthetic deoxyoligonucleotides as hybridization probes we isolated an exon coding for a portion of TGF-α from a human genomic DNA library. Utilizing this exon as a probe, a cell line derived from a human renal cell carcinoma was identified as a source of TGF-α mRNA. A cloned TGF-α cDNA was isolated from a cDNA library prepared using RNA from this cell line, and was found to encode a precursor polypeptide of 160 amino acids. The 50 amino acid mature TGF-α produced by expression of the appropriate coding sequence in E. coli binds to the epidermal growth factor receptor and induces the anchorage independence of normal mammalian cells in culture.</div>
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Human transforming growth factor-α: Precursor structure and expression in E. coli

Human transforming growth factor-α: Precursor structure and expression in E. coli

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri